I used to be able to use this with SourceTree. Now, I get a message 'no key found', despite the fact I have ~/.ssh/id_rsa defined, it's in my agent and Apple keychain, and I can use this fine everywhere else. Before, I could pick one of my ssh keys (I have dozens, but mainly use one for work and another for personal Git repos) during setup. It seems there is some change to how the setup and configuration of SSH keys work. I'm on a new project where I have to collaborate with some people on Windows and suggested they use SourceTree, and wanted to re-install SourceTree on my macs so I could help them learn this by demonstration, as I have used this from time to time in the past. I normally use the command line git client to work with GitHub or BitBucket.
I just rebuilt my laptop and iMac with clean installs of MacOS Sierra.